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Document Type |
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Thesis |
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Document Title |
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IDENTIFICATION OF DNA-CONTAINING EXOSOMES FORM PLASMA AND SERUM OF SAUDI MALES WITH TYPE II DIABETES تحديد الحمض النووي للإكسوزوم المستخلص من البلازما والمصل من الذكور السعوديين الذين يعانون من مرض السكري من النوع الثاني |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Type 2 diabetes (T2D) is a serious worldwide public health burden, which has reached epidemic proportions with an increasing prevalence. The disease leads to morbidity with the life expectancy being reduced, while additional implications include premature coronary heart disease, peripheral vascular disease, renal failure, stroke and amputation. New methods are desired to improve the diagnostic work-up. Exosomes (EXOs) are cell-derived vesicles displaying various proteins on their membrane surfaces. In addition, they are readily available in blood samples where they constitute potential biomarkers of human diseases, such as cancer. Here, we examine the potential of distinguishing diabetic patients from controls based on the differential levels of cellular exosomal proteins. In this study, epithelial cell adhesion molecule (EpCAM) positive EXOs were isolated from 30 diabetic males and 30 controls to quantify EXOs from the blood plasma samples. The presence of exosomal membrane bound proteins CD9 and CD63 were analyzed by flow-cytometry. The level of +CD63 EXOs and +CD9 EXOs were significantly higher in men with T2D (P= 0.04 and P= 0.001; respectively). The level of +CD63 EXOs was correlated positively and strongly with +CD9 EXOs in the diabetics and the controls (P= 0.0001). In the diabetic subjects, the level of +CD63 EXOs was positively correlated with glucose (P= 0.06). In the controls, +CD63 EXOs and +CD9 EXOs levels were correlated with HDL level (P= 0.04 and P= 0.03; respectively). The present study demonstrates that the levels of +CD63 EXOs and +CD9 EXOs are increased in patients with T2D, where they could contribute to disease progression. In general, exosomal biomarkers are still in the early discovery stage and their potential value in clinical diagnostics waits to be fully explored. |
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Supervisor |
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Prof. Rowyda Nawwaf Al-Harithy |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1439 AH
2017 AD |
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Added Date |
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Monday, October 23, 2017 |
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Researchers
| ناهد سويرح البلوي | Al-Blwi, Nahid Swerah | Researcher | Master | |
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