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Document Type |
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Thesis |
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Document Title |
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The Possible Protective Effect of L-Arginine in Experimentally Induced Necrotizing Enterocolitis in Rats الوقاية المحتملة لدواء ال- أرجينين في الالتهاب المعوى القولوني الناخر المستحدث تجريبيا في الجرذان |
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Subject |
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Faculty of Medicine > MEDICAL PHARMACOLOGY |
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Document Language |
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Arabic |
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Abstract |
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Necrotizing enterocolitis (NEC) is a leading cause of mortality and morbidity in the neonatal intensive care unit. Until now the etiology of NEC remains unclear, prevention and treatment strategies are often inadequate. Accordingly, a lot of research started to evaluate the effectiveness of some medications which contribute to the protection of premature infants from this deadly disease, L-arginine is one of these medications. The objective of the current study was to test the hypothesis that administration of L-arginine would have a protective effect in experimentally induced necrotizing enterocolitis in rats. The study was conducted on 46 male albino full-term rats which were divided into 3 main groups: Control group (10 rats), L-arginine group (12 rats), and the experimental group, which has been divided into 2 sub-groups (each group = 12 rats). Group A: they received Lipopolysaccharide (LPS) 2 mg/kg/day to induce NEC, Group B: they injected by L-arginine prior to the disease induced by LPS, asphyxia and cold stress. After the animals had been scarified, histological changes and apoptosis were evaluated, gene expression of both iNOS and IL-12 were evaluated. Results showed that there is significant increase by 121.95% and 113% respectively in the expression of iNOS gene and IL-12 gene produced by oral administration of L-arginine in a protective group, In addition, administration of L-arginine attenuated body weight, body temperature and the histological changes, which were altered by LPS/asphyxia/cold stress; Furthermore, it decreased the apoptosis index significantly compared to the LPS/asphyxia/cold stress, cold stress group. This study was able to demonstrate that L-arginine administration had significant protective effect against NEC, but further clinical studies are required on preterm infant to confirm these results. |
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Supervisor |
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Prof. Magda Mohamed Hagras |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1436 AH
2015 AD |
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Co-Supervisor |
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Prof. Omar Ibrahim Saadah |
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Added Date |
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Monday, May 4, 2015 |
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Researchers
| ندى صدقة عسلي | Asali, Nada Sadaka | Researcher | Master | |
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