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Document Type |
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Thesis |
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Document Title |
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Effect of Corchorusolitorius Leaves Aqueous Extract On Carbon Tetra Chloride-Induced Acute Hepatotoxicity in Rats تأثير المستخلص المائي لأوراق الملوخية على التسمم الكبدي الحاد المحدث برابع كلوريد الكربون في الفئران |
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Subject |
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Faculty of Human Sciences and Designs |
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Document Language |
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Arabic |
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Abstract |
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Objectives: This study was planned to determine the liver protective and therapeutic of aqueous extract of Corchorus olitorius L (AECO) against hepatotoxicity induced by Carbon Tetrachloride (CCl4) in rats. Methods: The antioxidant activity and phenolic profiles of aqueous extract and ethanolic extract (AECOand EECO) were analyzed by DPPH and HPLC-MS methods. A total of forty-eight male rats were distributed into eight groups (n=6). Group (G I) (control), G II and III normal rats administrated with 500 and1000 mg of AECO / kg, b.wt, respectively. G IV (positive control) was injected by CCl4 (0.5 ml/kg/b.wt) every three days for 2 weeks. Whereas G (V and VI) (Pretreatment), administrated AECO (500 and 1000 mg/kg, b.wt), respectively for 4 weeks then received CCl4 for more 2 weeks. Group VII and VIII (Post-treatment) received CCl4 as G IV then treated with both doses of AECO for 6 weeks. The daily feed intake, body weight and weight gain percent and feed efficiency ratio were evaluated twice weekly. Effect of AECO on liver function test, lipid peroxidation (LP) and glutathione peroxidase (GPx) level were also investigated. Results: Phytochemical analysis showed that AECO have stronger antioxidant potential than EECO. The results also observed that CCl4 induced significant, increase in: liver enzymes (AST, ALT and ALP), billirubin and MDA serum levels and decrease serum levels of albumin, total protein and antioxidant enzyme Gpx compared to GI. Meanwhile, G II and III showed no significant change compared to G I. While G (V and VI) showed significant improvement in all mentioned parameter compared to G IV. Also, G (VII and VIII) the high dose showed more significant enhancement compared to low dose. The histopathological study of liver supported that the two doses of AECO were markedly mitigated the toxicity especially high dose to near-normal. In conclusion: These results indicated that AECO could be acting as a natural liver preventive agent that reduced liver toxicity induced by CCl4, this may be due to its radical scavenging activity and rich in flavonoid and phenolic compounds. |
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Supervisor |
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Prof. Dr. Lobna AbdEl-Fattah Haridy |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Saturday, June 6, 2020 |
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Researchers
| رهام خلف الغامدي | Alghamdi, Reham Khlaf | Researcher | Master | |
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