Document Details

Document Type : Thesis 
Document Title :
“BIODEGRADATION OF PETROLEUM HYDROCARBONS BY HALOPHILIC BACTERIAL CONSORTIA ENRICHED FROM RED SEA JEDDAH, SAUDI ARABIA”
تحلل الهيدروكربونات النفطية من قبل اتحادات البكتيريا المحبة للملوحة من البحر الأحمر جدة، المملكة العربية السعودية
 
Subject : Faculty of Marine Sciences 
Document Language : Arabic 
Abstract : The present study, details about a halophilic bacterial consortium enriched from water and sediment samples from Red sea, Jeddah, Saudi Arabia analyzed for PAHs degradation potential under saline condition (40 g/L NaCl concentration). The bacterial consortium was able to growth in halophilic mineral salt medium with PAHs as sole carbon source. Different types of selected LMW PAHs such as phenathrene (PHN) and fluorine (FLU) at different concentrations (25, 50, 100, 200, 500 ppm) and pyrene (PY) from HMW PAHs at 50 and 100 ppm concentrations was used in the study under saline condition. The results recorded 90% degradation of phenanthrene and fluorene upto 500 ppm. HMW pyrene revealed 79% and 69% degradation at 50 ppm and 100 ppm concentration under saline condition by the halophilic consortium. Co-metabolic study performed by adding FLU with PY enhanced the biodegradation process and reduced the time required for biodegradation. Addition of FLU (100 ppm) along with the PY (50 ppm) recorded 85% of PY degradation in 8 days and 98% degradation in 12 days. Addition of yeast extract during PY degradation recorded complete degradation of PY (50 ppm) in 12 days. Lab Scale reactor study with CSTR (continuous stirred tank reactor) used to investigate the PAHs degradation and petroleum refinery wastewater treatment efficiency of in the halophilic bacterial consortium. The results recorded 95% COD removal in 32 days with complete degradation of LMW PAHs in 12 days under saline condition. HMW PAHs recorded 91% degradation in 28 days by the bacterial consortium. Bacterial strains in the consortium were identified by using molecular techniques such as DNA isolation and next generation sequencing. Phylogenetic analysis revealed Ochrobactrum sp dominated the PAHs degrading halophilic consortium. Next generation sequencing results of both PAHs degradation and reactor study contained the dominance of Ochrobactrum sp. Marinobacter sp occupied the second position in the consortium, followed by Pseudomonas sp and Stenotrophomonas maltophilia in third and fourth position. Thus the potential halophilic bacterial consortium can be recommended to be employed in the treatment of saline petroleum refinery wastewater 
Supervisor : Dr. Mamdoh Jamal 
Thesis Type : Master Thesis 
Publishing Year : 1439 AH
2018 AD 
Added Date : Monday, June 4, 2018 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
امينة جزاء الشيخALshaikh, Aminah JezaResearcherMaster 

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