Document Details

Document Type : Thesis 
Document Title :
Modulation of Doxorubicin Cytotoxicity by Resveratrol in Human Breast Cancer Cell Line
تعديل النشاط المضاد للسرطان لعقار الدوكسوروبيسين في خلايا الثدي البشرية السرطانية بواسطة مادة الريزفراترول
 
Subject : MEDICAL PHARMACOLOGIY 
Document Language : Arabic 
Abstract : Breast cancer is the most common cancer in the Arab world and it ranked first among Saudi females. Doxorubicin, an anthracycline antibiotic is one of the most effective anticancer agents used to treat breast cancer. Unfortunately, chronic cardiotoxicity is a major limiting factor of the chemotherapeutic use of doxorubicin. Therefore, our study was designed to assess the role of a natural product resveratrol on sensitization of human breast cancer cells to the action of doxorubicin in an attempt to minimize doxorubicin effective chemotherapeutic dose and thereby its side effects. Treatment of MCF-7 cells with 15 µg/ml resveratrol either simultaneously or 24 hours before doxorubicin increased the cytotoxicity of doxorubicin, with IC50 were 0.056 and 0.035 µg/ml, respectively compare to doxorubicin alone IC50 (0.417 µg/ml). Moreover, flow cytometric analysis of The MCF-7 cells treated simultaneously by doxorubicin (0.5 µg/ml) and resveratrol showed significant arrest of the cells in Go (83.26%) compare to 1.99% of doxorubicin alone. In addition, Cellular uptake of doxorubicin in the MCF-7 cells has been increased by almost 50%. On the other hand, when resveratrol is given 24 hours before doxorubicin although there was more increase in the cytotoxic effect of doxorubicin against the growth of the cells (IC50 = 0.035 µg/ ml) but there were less decrease in percentage arrest of Go cells, less inhibition of doxorubicin-induced apoptosis and decreased doxorubicin cellular uptake into the cells. In conclusion, resveratrol was cytotoxic by itself and it increased the cytotoxic activity of doxorubicin against the growth of human breast cancer cells when given either simultaneously or 24 hours before doxorubicin. 
Supervisor : DR. Abdel-Moneim M. Osman 
Thesis Type : Master Thesis 
Publishing Year : 1434 AH
2013 AD 
Co-Supervisor : Dr. Zoheir A. Damanhouri 
Added Date : Monday, March 11, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
هديل محمد بيوميBayoumi, Hadeel MohammedResearcherMaster 

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