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Document Type |
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Thesis |
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Document Title |
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In vitro Evaluation of Anti-tumor Activity of Doxorubicin Loaded-Nanoemulsion in Human Breast Cancer التقييم المعملي للنشاط المضاد للأورام للدوكسوروبيسين المحمل على مستحلب نانومتري في سرطان ثدي الإنسان |
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Subject |
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Science [Biochemistry] |
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Document Language |
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Arabic |
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Abstract |
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Doxorubicin (DOX) is an anticancer drug used to treat several cancer diseases. However, because of its poor bioavailability, hydrophobicity and cytotoxicity, it has several dose limitation aspects. In this study, five nanoemulsion formulations, containing soya phosphatidylcholine (SPC) / polyoxyethylenglycerol trihydroxystearate 40 (EU)/sodium oleate (SO) as surfactant, cholesterol (CHO) as oil phase and aqueous buffer, were produced. The droplet sizes, measured by the transmission electron microscope, for the blank nanoemulsion carriers were between 2.9 and 6.4 nanometer while the droplet sizes for the DOX-loaded nanoemulsions were significantly higher and were found to be in the range of 7.7-15.9 nm. The evaluation of both blank and DOX-loaded nanoemulsion formulations proved that the nanocarrier had improved the DOX efficacy and reduced its cytotoxicity. It showed that the cell growth inhibition of the breast cancer cell lines (MCF-7) have exceeded the commercial DOX by a factor of 1.7 with increased apoptosis activity and minimal cytotoxicity against the normal human foreskin cell line HFS of 28%. In contrast, commercial DOX was found to exhibit a significant non-selective toxicity against both (MCF-7) and (HFS) cell lines. In conclusion, we have developed a nanoemlsions formulation of doxorubicin with extremely small droplet sizes, which selectively and significantly inhibited cell proliferation of MCF-7 cells and increased apoptosis, thus improving the therapeutic potential for nanoemulsions as an anti-cancer drug delivery system. |
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Supervisor |
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Dr. Mayson Husni AlKhatib |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Co-Supervisor |
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Dr. Sawsan Hassan Mahassni |
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Added Date |
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Monday, April 2, 2012 |
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Researchers
| حياة مذكر البيشي | Albishi, Hayat Methker | Researcher | Master | |
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