Document Details

Document Type : Thesis 
Document Title :
Bioremediation of Oil Spill in Sea Shores of Saudi Arabia
المعالجة البيولوجية للملوثات النفطية في بعض شواطئ المملكة العربية السعودية
 
Subject : Sciences Faculty for Girls 
Document Language : Arabic 
Abstract : Kingdom of Saudi Arabia is one of the biggest countries in producing and exporting petroleum. So it has become prone to increasing the scope of oil pollutants which is started to become more clear about their bad effect to the natural environment and being dangerous to human health. It was very important to study the biodegradation effect on oil spills defending on the activation the normal flora of a natural environment or by the action of active selective microorganisms which able to degrade these pollutant. The location under study was Marduma bay, at the eastern Saudi Arabia shore. It was chosen to study this location as a part of oil spill of Arabian Gulf region. In this study, the Total viable microorganisms were counted in non contamination soil, contamination soil and rhizosphere. The higher concentration of microorganisms were recorded as follow; gram positive bacteria in non contamination soil, gram negative bacteria and fungi in contamination soil and actinomycetes in rhizosphere. Seventy two from Representative colonies were isolated, identified to the genes level. All active strains were studied for their ability to degrade crude oil and its fractions alkans fractions were followed by G.C. analysis. The most active strains was use to study the factors affecting the biodegradation activities. It was Bact. strain No.4 which able to degrade a bout 80.8 % of crude oil during 21 days at pH 7.2 and in presence of 1% of nitrogen fertilizers (NaNO3) and 0.8% of (K2HPO4) as phosphorous fertilizers. An applied experiment was design by polluting a sandy soil with 2% crude oil and were tested to study the effect of microbial seeding by the following; 1- The most active Bact.No.4. 2- A mixed culture of Bact.No.4, white rot and Streptomyces. 3- seeding by the total flora. Which made a removement of 99% of crude oil, 86.5% of hydrocarbons and 28.8% of aromatic at the end of 63 days of the incubation process. It was recorded the efficacy of microbial seeding to degrade oil and its fraction. 
Supervisor : Dr. Kawther El said Fahmi El Shinnawi 
Thesis Type : Master Thesis 
Publishing Year : 1431 AH
2010 AD 
Added Date : Sunday, July 4, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
شذى فاروق مرادmorad, Satha faroukResearcherMaster 

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