Document Details

Document Type : Article In Journal 
Document Title :
The use of flow cytometry to examine calcium signalling by TRPV1 in mixed cell populations
استخدام قياس التدفق الخلوي لفحص إشارات الكالسيوم بواسطة TRPV1 في تجمعات الخلايا المختلطة
 
Subject : Laboratory 
Document Language : English 
Abstract : Flow cytometric analysis of calcium mobilisation has been in use for many years in the study of specific receptor engagement or isolated cell:cell communication. However, calcium mobilisation/signaling is key to many cell functions including apoptosis, mobility and immune responses. Here we combine multiplex surface staining of whole spleen with Indo-1 AM to visualise calcium mobilisation and examine calcium signaling in a mixed immune cell culture over time. We demonstrate responses to a TRPV1 agonist in distinct cell subtypes without the need for cell separation. Multi parameter staining alongside Indo-1 AM to demonstrate calcium mobilization allows the study of real time calcium signaling in a complex environment. 
ISSN : 0003-2697 
Journal Name : Analytical Biochemistry 
Volume : 527 
Issue Number : 1 
Publishing Year : 1438 AH
2017 AD 
Article Type : Article 
Added Date : Monday, January 20, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مشرف بكري عساسAssas, Mushref BInvestigatorDoctoratemassas@kau.edu.sa
وسام هـ عبدالعالAbdulaal, Wesam HResearcherDoctoratewhabdulaal@kau.edu.sa
ماجد حمدي واكدWakid, Majed HResearcherDoctoratemwakid@kau.edu.sa
هيثم أ زكائيZakai, Haytham AResearcherDoctoratehzakai@kau.edu.sa
جليل أ ميانMiyan, Jaleel AResearcherDoctoratej.miyan@manchester.ac.uk
جوان ل بينوكPennock, Joanne LResearcherDoctoratejoanne.l.pennock@manchester.ac.uk

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