Document Details
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Document Type |
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Thesis |
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Document Title |
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Phytochemical and Biological Study of Euphorbia cuneata دراسة كيميائية وبيولوجية لنبات يوفوربيا كيوناتا |
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Subject |
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faculty of Pharmacy |
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Document Language |
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Arabic |
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Abstract |
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The aim of current study was to isolate and identify active metabolites of Euphorbia cuneata (family Euphorbiaceae) and assess their cytotoxic activity on three
different cell lines: MCF-7 (breast cancer), HepG2 (hepatocellular carcinoma), and
HCT-116 (colon cancer). Phytochemical study of the methanolic extract of Euphorbia cuneata aerial parts using different chromatographic techniques led to the isolation of two new compounds: named cyclocuneatol (2) and cuneatannin (11), along with nine known metabolites: β-sitosterol (1), 3β-hydroxycycloart-25-ene-24-one (3), cycloart-25-ene-3β,24β-diol (4), cycloart-23Z-ene-3β,25-diol (5), 2R-naringenin (6), β-sitosterol-3-O-β-D-glucopyranoside (7), quercetin (8), kaempferol-7-O-β-glucoside (9), and 2R-naringenin-7-O-β-glucoside (10). Their structures were established on the basis of physical, chemical and spectral data (UV, IR, 1D (1H and 13C NMR) and 2D NMR (1H-1H COSY, HSQC, HMBC and NOESY), as well as HRESIMS and comparison with literature data. Their cytotoxic activity was assessed towards HepG2, MCF7, and HCT116 cancer cell lines by sulphorhodamine B test (SRB). It is noteworthy that 3 and 4 demonstrated the most significant activity towards HepG2, MCF7, and HCT116 cells (IC50 2.6, 2.7, and 2.4 μM, respectively for 3 and 3.4, 4.1, and 5.3 μM, respectively for 4) in comparison with doxorubicin (IC50 0.18, 0.6, and 0.2 μM, respectively). |
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Supervisor |
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Prof. Dr. Hossam Mohamed Abdallah |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Monday, May 18, 2020 |
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Researchers
| أمجد أحمد الغامدي | Alghamdi, Amjad Ahmed | Researcher | Master | |
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