Document Details

Document Type : Thesis 
Document Title :
EFFECT OF CURCUMIN ON HEAD AND NECK CANCERS CELLS GROWTH
تأثير الكوركمين على نمو خلايا سرطانات الرأس والرقبة
 
Subject : faculty of science 
Document Language : Arabic 
Abstract : Head and neck cancer (HNC) generally stated as a span of malignant neoplasms that originate from soft tissue. HNC is considered the 7th most frequent type of cancers in the world and the 3rd most common cancers in Saudi Arabia. The current chemotherapy and radiotherapy regimes used to treat cancer show adverse side effects. Natural products are generally safe, effective and less expensive substitutes of anticancer chemotherapeutics. Based on the previous studies for its potential therapeutic uses, curcumin is the major active compound of the turmeric plant, may be used as a good natural therapeutic agent in the prevention and treatment of cancer. It has been recently demonstrated that the chemo-preventive activities of curcumin might be due to its ability to increase DNA damage, apoptosis and cell cycle arrest. The present study aimed to investigate the effect of curcumin on the proliferation of HNO97 cells. The cells were cultured and treated with different concentrations of curcumin, and WST-1 assay was carried out for evaluating the IC50 value after 24 h of treatment. We found that curcumin significantly inhibit the growth of HNO97 in a time and dose-dependent manner (IC50=35μM). Flow cytometry data revealed that curcumin did not alter the cell cycle progression of HNO97 cells, but led to apoptosis in a time-dependent manner. Data from comet assay showed that upon treatment of cells with curcumin, the significant DNA damage was found as evident from comet tail. Finally, curcumin treatment suppressed colony formation capacities of HNO97 cells. Overall findings demonstrate that curcumin inhibits the proliferation and induces apoptosis in HNO97 cells. Our study suggests that curcumin can be developed as a natural therapeutic agent for the management of HNC. 
Supervisor : Dr. Mazin Abdulaziz Zamzami 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2020 AD 
Co-Supervisor : Dr. Samar Abdullah Damiati 
Added Date : Thursday, March 12, 2020 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
زهور خلف الله المالكيAl-Malki, Zuhur Khalaf AllahResearcherMaster 

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