Document Details
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Document Type |
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Thesis |
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Document Title |
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EVALUATED EFFECT AGE OF MOTHERS ON TELOMERES LENGTH FROM MESENCHYMAL STEM CELLS ISOLATED FROM HUMAN FETAL MEMBRANES تقييم تأثير عمر الأمهات على أطوال تيلوميرات الخلايا الجذعية الوسيطة المعزولة من الأغشية الجنينية البشرية |
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Subject |
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faculty of science |
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Document Language |
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Arabic |
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Abstract |
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Age-related cellular changes and limited replicative capacity of adult mesenchymal stem cells (MSCs) are of the challenges confronting stem cell research. For that, the MSCs from human fetal membranes (hFM-MSCs), including placental, umbilical cord, and amniotic membrane, are considered a great alternative to adult MSCs. However, the effect of mothers' age on hFM-MSC cellular properties is still not clearly established. This study aimed to evaluate the effect of mothers' age on hFM-MSC telomere length, telomerase activity, and proliferation ability in three different age groups: GI (20–29 years): GII (30–39 years), and GIII (≥ 40 years). Human fetal membranes FM samples were collected from pregnant women age ≤ 37 weeks after obtaining consent. hFM-MSCs were isolated and cultured to characterise them by flow cytometry and assess proliferation by MTT assay and doubling time. Telomere length and expression levels of human telomerase reverse transcriptase (hTERT) were assessed by qRT-PCR and MMq-PCR respectively. hFM-MSCs in the three age groups were spindle-shaped, plastic-adherent, and exhibited high proliferation rates and strong expression of hMSC markers. GI showed the longest telomere length in hMSCs in various FM areas, while GIII showed the highest level of telomerase expression. There was no difference in telomere length between GII and GIII, and both groups showed the same hMSC characteristics. In conclusion, although the hFM-MSCs derived from different fetal membranes maintained the MSC characteristics in all study groups, the hFM-MSCs of older mothers had shorter telomeres but higher telomerase activity and proliferation rate than did those derived from younger mothers; this might be attributed to the decreased telomere length in older mothers or the growth factors (b-FGF) used in hMSCs culture. Thus, the hFM-MSCs of older mothers could be unsuitable for expansion in vitro or stem cell therapy. Determination of telomere length and telomerase expression level of hFM might help characterise and understanding the biological differences of hFM-MSCs in different age groups.
Age-related cellular changes and limited replicative capacity of adult mesenchymal stem cells (MSCs) are of the challenges confronting stem cell research. For that, the MSCs from human fetal membranes (hFM-MSCs), including placental, umbilical cord, and amniotic membrane, are considered a great alternative to adult MSCs. However, the effect of mothers' age on hFM-MSC cellular properties is still not clearly established. This study aimed to evaluate the effect of mothers' age on hFM-MSC telomere length, telomerase activity, and proliferation ability in three different age groups: GI (20–29 years): GII (30–39 years), and GIII (≥ 40 years). Human fetal membranes FM samples were collected from pregnant women age ≤ 37 weeks after obtaining consent. hFM-MSCs were isolated and cultured to characterise them by flow cytometry and assess proliferation by MTT assay and doubling time. Telomere length and expression levels of human telomerase reverse transcriptase (hTERT) were assessed by qRT-PCR and MMq-PCR respectively. hFM-MSCs in the three age groups were spindle-shaped, plastic-adherent, and exhibited high proliferation rates and strong expression of hMSC markers. GI showed the longest telomere length in hMSCs in various FM areas, while GIII showed the highest level of telomerase expression. There was no difference in telomere length between GII and GIII, and both groups showed the same hMSC characteristics. In conclusion, although the hFM-MSCs derived from different fetal membranes maintained the MSC characteristics in all study groups, the hFM-MSCs of older mothers had shorter telomeres but higher telomerase activity and proliferation rate than did those derived from younger mothers; this might be attributed to the decreased telomere length in older mothers or the growth factors (b-FGF) used in hMSCs culture. Thus, the hFM-MSCs of older mothers could be unsuitable for expansion in vitro or stem cell therapy. Determination of telomere length and telomerase expression level of hFM might help characterise and understanding the biological differences of hFM-MSCs in different age groups. |
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Supervisor |
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Prof. Saleh Abdulaziz Alkarim |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Co-Supervisor |
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Prof. Hassan Salah Abduljabbar |
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Added Date |
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Thursday, January 23, 2020 |
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Researchers
| غدير إبراهيم الرفاعي | AL-Refaei, Ghadeer Ibrahim | Researcher | Doctorate | |
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