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Document Type |
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Thesis |
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Document Title |
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The Efficacy of Plant Essential Oil Nanoemulsions on some Pathogen and Dendritic cells فاعلية مستحلب النانو للزيوت العطرية النباتية على الميكروبات والخلايا المناعية الشجرية |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Dendritic cells (DCs) are specialized antigen-presenting cells that orchestrate the innate and adaptive immune response. Pathogenic microbes remain a major health problem because they are responsible for causing many diseases. Aim: The aim of this study was to prepare nanoemulsions (NEs) that would work as new therapeutic agents against pathogenic microbes and to investigate NEs role in controlling DCs phenotype expression, apoptosis, and cytokine secretion. Method: Three NEs were produced by mixing essential oils, surfactants and water with droplet sizes of NEs formulations in the range of 25-62nm. DCs were generated in vitro from peripheral blood mononuclear cells of healthy donors, followed by stimulation with lipopolysaccharide (LPS), Vitamin D and NEs concentrations. DCs morphology was determined using bright field inverted microscope. Phenotype characteristics and apoptosis were investigated using flow cytometry, and ELISA technique to determine IL-10 and IL-12(p70) production. Antimicrobial activities of the three NEs were examined against some pathogenic Gram positive, negative bacteria and yeast. Results: NEs showed significantly lower expression of maturation marker CD83 compared to LPS stimulated DCs. Also, costimulatory molecules CD80 showed higher expression and CD86 showed significantly lower expression compared with unstimulated DCs. In addition, NEs showed minor not significant effect on the viability and apoptosis of DCs at high concentrations, beside secretion significantly low levels of IL-12(p70) and high levels of IL-10. NEs showed no toxic effect against Artemia salina except flaxseed (Linum usitatissimum) NE. On the other hand, NEs showed antimicrobial activity against all tested microorganism. The best MIC of flaxseed (Linum usitatissimum) NEs was 10.93 μg/ml against Pseudomonas aeruginosa, for Coriander (Coriandrum sativum) NEs was 15.70 μg/ml against Staphylococcus aureus, and for Dill (Anethum graveolens) NEs was 21.09 μg/ml against Pseudomonas aeruginosa. Conclusion: Our data suggested that NEs may have tolerogenic effect on DCs and antimicrobial activity against the tested pathogenic microbes. |
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Supervisor |
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Prof. Dr. Magda Mohamed Aly |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Co-Supervisor |
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Dr. Alia Mohammed Aldahlawi |
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Added Date |
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Monday, May 29, 2017 |
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Researchers
| غيداء سامي صديق | Siddiq, Ghaida Sami | Researcher | Master | |
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