Document Details
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Document Type |
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Thesis |
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Document Title |
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In vivo evaluation of the antitumor activity of a molecular-targeted agent encapsulated with the atorvastatin in a microemulsion التقييم الحيوي للنشاط المضاد للسرطان لعامل مستهدف للجزيئات مغلف مع أتورفاستاتين في مستحلب مايكرومتري |
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Subject |
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Faculty of Sciences > Biochemistry department |
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Document Language |
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Arabic |
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Abstract |
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Gemcitabine (GEM), a well-tolerated chemotherapeutic agent, and atorvastatin (ATV), a cholesterol lowering agent, are mostly effective in cancer treatment when formulated in combination regimens. The aim of this study was to evaluate the antitumor activity of the microemulsion (ME) formulations containing GEM in combination with ATV in Ehrlich ascites carcinoma (EAC) bearing Swiss albino mice. The antitumor activity of the studied formulas in EAC-bearing mice was evaluated by detecting the efficacy of the administered drug through measuring the median survival time (MST). The side effects of the subjected formulas on the heart, liver and kidney were assessed. 220 mice were divided into eleven groups (n=20). Groups I and II served as the negative (normal) and positive (EAC-bearing mice) controls, respectively. Groups III-XI were EAC-bearing mice treated with blank-ME, 30mg of GEM- loaded ME, 60mg of GEM dissolved in water (GEM-Sol), 10mg of ATV-loaded ME, 20mg of ATV-loaded ME, 10mg of ATV dissolved in water, 20 mg of ATV dissolved in water, a combination of 30mg of GEM and 10mg of ATV-loaded ME (GEM+ATV/LME) and a combination of 30mg of GEM and 10mg of ATV dissolved in water, respectively. The entire drug formulas were given orally, except GEM-Sol was intraperitoneally injected into the mice. Mice treated with GEM+ATV/LME, which has z-average diameter of 111.9±23.28 nm and zeta potential of -28.6 ±3.35 mV, has MST of 28.0±3.85day. GEM+ATV/LME did not cause hematological toxicity, hepatotoxicity, nephrotoxicity and cardiotoxicity compared to the administered combination treatment, dissolved in water, and the single treatments of ATV and GEM, either dissolved in water or ME. In conclusion, incorporating ATV into GEM in ME formulation has reduced the side effects of GEM which could make it a promising combination formula. |
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Supervisor |
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Dr. Mayson Husni Hasan Alkhatib |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1437 AH
2016 AD |
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Co-Supervisor |
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Dr. Hanan Saeid Omar Alnahdi |
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Added Date |
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Monday, April 25, 2016 |
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Researchers
| جمعه فيتل السلمي | Al sulami, Jomaa Feital | Researcher | Master | |
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