Document Details

Document Type : Article In Journal 
Document Title :
Nanodiamonds as an effective and novel matrix for immobilizing β galactosidase
Nanodiamonds as an effective and novel matrix for immobilizing β galactosidase
 
Document Language : English 
Abstract : The present study demonstrates the biospecific adsorption of Aspergillus oryzae β galactosidase on glutaraldehyde functionalized nanodiamonds (NDs). Transmission electron microscopy showed that the synthesized NDs were of 20 nm size. Enzyme activity retained as a result of immobilization was 7420 U/gm of modified NDs. The optimal pH and temperature for soluble and immobilized β galactosidase was observed at pH 4.5 and at 50 °C, respectively. However, significant stability was observed at both higher and lower limits of pH and temperature for the enzyme immobilized on glutaraldehyde modified NDs. Moreover, our findings demonstrated that β galactosidase immobilized on surface functionalized NDs retained greater biocatalytic activity even after 2 months of storage and at higher galactose concentration, and upon repeated uses as compared to enzyme in solution. Modified NDs bound β galactosidase showed improved hydrolysis of lactose from solution in batch processes at various temperatures even after 10 h, thereby suggesting its use for hydrolyzing lactose in dairy products. 
ISSN : 0960-3085 
Journal Name : Food and Bioproducts Processing 
Volume : 95 
Issue Number : 0 
Publishing Year : 1436 AH
2015 AD 
Article Type : Article 
Added Date : Sunday, April 10, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Shakeel Ahmed AnsariAnsari, Shakeel AhmedInvestigator  
Rukhsana SatarSatar, Rukhsana Researcher  
Syed Kashif ZaidiZaidi, Syed KashifResearcher  
Muhammad Imran NaseerNaseer, Muhammad ImranResearcher  
Sajjad KarimKarim, Sajjad Researcher  
Mohammad Husein AlqahtaniAlqahtani, Mohammad HuseinResearcher  
Mahmood RasoolRasool, Mahmood Researcher  

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