Document Details

Document Type : Article In Journal 
Document Title :
Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis
Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis
 
Document Language : English 
Abstract : The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, Km was increased nearly three-folds for MβG while Vmax for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products. 
ISSN : 1359-5113 
Journal Name : Process Biochemistry 
Volume : 74 
Issue Number : 12 
Publishing Year : 1433 AH
2012 AD 
Article Type : Article 
Added Date : Sunday, April 3, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Shakeel Ahmed AnsariAnsari, Shakeel AhmedInvestigator  
Rukhsana SatarSatar, Rukhsana Researcher  
Fahad AlamAlam, Fahad Researcher  
Mohammed Husein AlqahtaniAlqahtani, Mohammed HuseinResearcher  
Adeel Gulzar ChaudharyChaudhary, Adeel GulzarResearcher  
Muhammad Imran NaseerNaseer, Muhammad ImranResearcher  
Sajjad KarimKarim, Sajjad Researcher  
Ishfaq Ahmed SheikhSheikh, Ishfaq AhmedResearcher  

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