Document Details

Document Type : Thesis 
Document Title :
Studies on The Antiproliferative and Antioxidant Activities of Rhazya stricta Alkaloids on Cultured Colon Cancer Cells
دراسات عن الأنشطة المضادة للنمو والمضادة للأكسدة لقلويدات نبات الحرمل على خلايا سرطان القولون المزروعة
 
Subject : Faculty of Sciences > biological sciences department 
Document Language : Arabic 
Abstract : Colon cancer is the third most common cancer and the fourth leading cause of cancer related deaths worldwide. Natural products have been used for the prevention and treatment of human diseases in a traditional medicine. The molecular mechanisms by which natural products extract inhibit human cancer cell growth are essentially unknown. Rhazya stricta Decne is an important folk medicinal plant. It has anti-oxidant, anti-carcinogenic, and free radical scavenging properties. The objectives of present study is to investigate the anti-proliferative and antioxidant activities of Rhazya stricta Decne strong basic alkaloid extract (RSSBA) on human colon cancer cells (HCT116). The antiproliferative and cytotoxic activities of the RSSBA were determined using the trypan blue dye exclusion and the WST-1 cell proliferation tests. The underlying possible mechanism of cell death was investigated whether it is necrotic or apoptotic by single cell gel electrophoresis (comet assay) and by assaying for caspase 3 / 7 and caspase 8 activities. The antioxidant activities of the RSSBA were investigated by determining the total antioxidant capacity (TAC), catalase activities and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability. Results showed that RSSBA inhibited human colon cancer cells proliferation and induced apoptosis and that apoptotic activities are associated with caspase 3 / 7 and independent of caspase 8. Also, results showed that RSSBA extract alone had total antioxidant capacity TAC, catalase and DPPH activities. The extract antioxidant activities were demolished when tested in HCT116, possible implication for the presence of antioxidant counter activities in cancer cells. 
Supervisor : Prof. Sufian Mohammad El-Assouli 
Thesis Type : Master Thesis 
Publishing Year : 1437 AH
2016 AD 
Co-Supervisor : Dr. Abdulkader M. Shaikh Omar 
Added Date : Monday, February 8, 2016 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
غادة عبد الجليل صابرsaber, Ghadah Abdul JalelResearcherMaster 

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