Document Details

Document Type : Thesis 
Document Title :
Immunological and histological studies of stem cells in the human placenta and umbilical cord
دراسات مناعية ونسيجية للخلايا الجذعية في المشيمة والحبل السري للإنسان.
 
Subject : Faculty of Sciences > biological sciences department 
Document Language : Arabic 
Abstract : Although the human placenta and umbilical cord are medical wastes, they became the main sources of stem cells. Their easy isolation and ability to resist immune rejection, and their ability to differentiate in to different types of adult cells made them superior to other sources of stem cells. The main objectives of the present study was to identify the precise anatomical localization of mesenchymal (MSCs) and hematopoietic (HSCs) stem cells in different regions of placenta and umbilical cord and which of those areas are rich in such cells, and to determine the effect of mother age on the presence and strength of their specific stem cells marker expression. Full term placentae (n=100) of the following mother ages were used in the study: Group A(20-25 years );Group B(25-30 years );Group C(30-35 years);group D(35-40 years), Group E ( 40 and more years) Immunohistochemical techniques for CD105,CD29 markers for mesenchymal stem cell and CD34 marker for hematopoietic stem cell were used to localize and study the area percent of markers expression in tissues from all age groups. Using SPSS program. Microscopic examination showed strong immunoexpression of (MSCs) in maternal decidual membrane and villus syncytiotrophoblast compared to the 4 layers of fetal amniotic membrane. High expression in chorionic villi was more than fetal membrane. (HSCs) were found to be only localized in the fetal capillary endothelium of the both (free & stem chorionic villi). Regarding umbilical cord strong immunoexpression of (MSCs) were found at Wharton's jelly cell and amniotic membrane A striking finding was that, It was observed that the lining endothelial of fetal capillaries and umbilical cord vessels give positive expression for both mesenchymal cell markers ( CD105,CD29) as well as the marker of hematopoietic cells (CD34). Those cells could be considered as an intermediate type-carrying characteristic of both cells. They were named in literature (endothelial pro-generation stem cells).Results also pointed to the effect of mother age on the presence and intensity of marker expression characterized different types of stem cells. Decrease in expression of those immunohistochemical markers was observed in placentae and umbilical cord as mother aged. 
Supervisor : Prof. Saleh Abdulaziz Alkarim 
Thesis Type : Master Thesis 
Publishing Year : 1435 AH
2014 AD 
Co-Supervisor : dr. Soad Shaker Ali 
Added Date : Tuesday, February 25, 2014 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
غدير إبراهيم الرفاعيAL-refaei, Ghadeer IbrahimResearcherMaster 

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