Document Details

Document Type : Thesis 
Document Title :
Ultrastructural Studies on The Toxic Changes Induced by Methyl Tertiary Butyl Ether on Mice Ovary and The Possible Protective Role of Vitamin E
دراسات تركيبية دقيقة للتغيرات السامة التي يحدثها مركب ميثيل ثلاثي بيوتيل الإيثر في مبيض الفئران والدور الوقائي المحتمل لفيتامين هـ
 
Subject : Faculty of Sciences 
Document Language : Arabic 
Abstract : Methyl-tert-butyl ether (MTBE) is a synthetic organic compound added to gasoline in Saudi Arabia. The leakage of MTBE contaminates the ground water and consequently the drinking water. The present study focused on the effect of MTBE in drinking water on the ovarian structure and function of Swiss albino mice. The mice were divided into eight groups treated via stomach tube as follows: first group (control) administered olive oil (the vehicle of vitamin E), second group treated with vitamin E (100 mg/Kg), three groups treated with MTBE (1000, 2000, 4000 ppm, respectively) for 60 days and another three groups treated with similar concentrations of MTBE besides vitamin E for the same duration. Blood samples were collected for the measurement of the blood electrolytes, progesterone and estrogen levels in sera of all animals. The results showed significant decrease in magnesium, calcium and progesterone. However, treatment with vitamin E and MTBE revealed incomplete restoration of the previously measured parameters. Ovarian tissues were prepared for light and electron microscopy. The results showed a reduction in the growing follicles, the Graafian follicles and the corpus luteum in all groups treated with MTBE in a dose dependent manner. However, treatment with vitamin E and MTBE revealed incomplete restoration of the previously measured parameters. Light microscopic studies revealed deformation and damage of different oocytes and follicle cells in MTBE treated mice, while electron microscopy revealed mitochondrial and nuclear changes, besides the formation of fat droplets and lysosomal structures as well as cytoplasmic vacuoles. These changes were decreased in the ovarian tissues of mice treated with MTBE plus vitamin E. It could be concluded that MTBE lead to severe alterations in the ovarian structure. Future studies are recommended on the effect of MTBE on testicular tissues as well as other body organs. 
Supervisor : Prof Osama A. Abu-zinadah 
Thesis Type : Doctorate Thesis 
Publishing Year : 1432 AH
2011 AD 
Co-Supervisor : Prof. Hussein K. H. Ali . Prof. Tarek R. R. Moharrem 
Added Date : Monday, June 27, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد على الفقيهAl- Fakih, Mohammed AliResearcherDoctorate 

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