Document Details

Document Type : Article In Journal 
Document Title :
some cultural conditions for maximum bioextraction of grapefruit waste pactin
some cultural conditions for maximum bioextraction of grapefruit waste pactin
 
Subject : some cultural conditions for maximum bioextraction of grapefruit waste pactin 
Document Language : English 
Abstract : Bioextraction of pectin from grapefruit waste by Kluyveromyces marxianus was markedly influenced by the age of seed culture, inoculum size and medium volume! flask (aeration), as well as the pH value of the fermentation. Thus, 24h old seed culture at inoculum size of 4% and 60 ml medium/250ml Erlenmeyer flask and pH value of 6, were responsible for maximum pectin extraction (about 99% of that originally contained in GFW), by the tested yeast. The culture filtrate was found to have some hydrolytic enzymes, The major enzyme activities resided in endopolygalacturonase followed by protopectinase. While cellulase activity was nearly absent and very low amylase activity was also detected. On the other hand, glucoamylase activity was not detected. The characterization of both microbiologically and chemically extracted pectin samples indicated that the former had higher percentages of methoxyl groups, higher degree of estrification and higher free carboxyl groups, but lower values of galacturonic acid and thus possesses superior qualities to chemically extracted pectin 
ISSN : 1012-1319 
Journal Name : Science Journal 
Volume : 2 
Issue Number : 3 
Publishing Year : 1426 AH
2006 AD 
Article Type : Article 
Added Date : Tuesday, January 25, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
صالح محمد القرنيalqurni, saleh muhammedInvestigatorDoctorate 

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