تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Effect of thawing rate and post-thaw culture on the cryopreserved fetal rat islets: functional and morphological correlation
Effect of thawing rate and post-thaw culture on the cryopreserved fetal rat islets: functional and morphological correlation
 
لغة الوثيقة : الانجليزية 
المستخلص : The ability of the fetal pancreatic islet cells to multiply rendered them a potential tissue for transplantation studies to cure diabetes. A bank of fetal islets could be created with proper storage in liquid nitrogen. The aim of this study is to evaluate the effect of thawing rate and post-thaw culture on the structural and functional integrity of isolated cryopreserved islets of rat fetuses. Fetal rat islets were isolated by the collagenase digestion, cultured for three days, and then cryopreserved using dimethylsulphoxide as cryoprotectant and the step-rate cooling to -40 degrees C before immersing them in liquid nitrogen. The islets were thawed by the slow or fast warming rates using hyperosmolar sucrose solution and then cultured for 1 or 2 days. Insulin and C-peptide contents of the slow thawed islets were higher than those of the control. In the fast thawed islets the contents were similar to those of the control. Insulin and C-peptide release in response to glucose for the slow thawed islets were lower than those of the control and in the fast thawed islets they were similar to that of the control. Histological examination showed irregular periphery and fragmented central part of the large slowly thawed islets, which showed also variable immunohistochemical reaction to anti-insulin serum, ranging from strongly positive reaction to markedly weak reaction. Fast thawed islets showed mostly regular periphery and their reaction to the anti-insulin serum was slightly weaker than that of the control islets. It was concluded that fast thawing and post-thaw culture is much better than slow thawing, as indicated by nearly normal insulin and C-peptide content and release and intact structural integrity 
ردمد : 0024-3205 
اسم الدورية : Life Sciences 
المجلد : 78 
العدد : 17 
سنة النشر : 1426 هـ
2006 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Monday, April 4, 2011 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
فيصل المشاطAl-Mashat, Faisal باحث مشاركدكتوراه 
عبدالرحمن صبيانيSibiany, AbdulRahman باحث مشاركدكتوراه 
محمد العرضاويArdawi, Mohammed باحث مشاركدكتوراه 
محمد باداودBadawoud, Mohammed باحث مشاركدكتوراه 

الملفات

اسم الملفالنوعالوصف
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